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mouse monoclonal anti en1 antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse monoclonal anti en1 antibody
    Immunofluorescence of hESC-derived mDA progenitors at day 16 (A) LMX1 (floorplate marker) and FOXA2 (ventral midbrain marker), (B) LMX1 and OTX2 (forebrain and midbrain marker), (C) LMX1 and <t>EN1</t> (caudal ventral midbrain marker), (D) LMX1 and NGN2 (neurogenic maker), and (E) SOX2 (neural progenitor marker) and DCX (immature neuronal marker). On day 16, most cells are LMX1 + , FOXA2 + , OTX + 2 and EN1 + mDA progenitors (A-C). Few cells expressed NGN2 (D) and DCX (E). Scale bars, 100 μm.
    Mouse Monoclonal Anti En1 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+en1/anti-Engrail-1/pmc10511858-3-0-6
    Average 93 stars, based on 38 article reviews
    mouse monoclonal anti en1 antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons"

    Article Title: A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2023.102355

    Immunofluorescence of hESC-derived mDA progenitors at day 16 (A) LMX1 (floorplate marker) and FOXA2 (ventral midbrain marker), (B) LMX1 and OTX2 (forebrain and midbrain marker), (C) LMX1 and EN1 (caudal ventral midbrain marker), (D) LMX1 and NGN2 (neurogenic maker), and (E) SOX2 (neural progenitor marker) and DCX (immature neuronal marker). On day 16, most cells are LMX1 + , FOXA2 + , OTX + 2 and EN1 + mDA progenitors (A-C). Few cells expressed NGN2 (D) and DCX (E). Scale bars, 100 μm.
    Figure Legend Snippet: Immunofluorescence of hESC-derived mDA progenitors at day 16 (A) LMX1 (floorplate marker) and FOXA2 (ventral midbrain marker), (B) LMX1 and OTX2 (forebrain and midbrain marker), (C) LMX1 and EN1 (caudal ventral midbrain marker), (D) LMX1 and NGN2 (neurogenic maker), and (E) SOX2 (neural progenitor marker) and DCX (immature neuronal marker). On day 16, most cells are LMX1 + , FOXA2 + , OTX + 2 and EN1 + mDA progenitors (A-C). Few cells expressed NGN2 (D) and DCX (E). Scale bars, 100 μm.

    Techniques Used: Immunofluorescence, Derivative Assay, Marker

    Immunofluorescence of hESC-derived mDA neurons at day 56 (A–C) (A) FOXA2 and TH (DA neuron marker), (B) NURR1 (DA neuron and post-mitotic DA neuroblast marker) and TH, (C) and EN1 and TH. Scale bars, 200 μm.
    Figure Legend Snippet: Immunofluorescence of hESC-derived mDA neurons at day 56 (A–C) (A) FOXA2 and TH (DA neuron marker), (B) NURR1 (DA neuron and post-mitotic DA neuroblast marker) and TH, (C) and EN1 and TH. Scale bars, 200 μm.

    Techniques Used: Immunofluorescence, Derivative Assay, Marker


    Figure Legend Snippet:

    Techniques Used: Recombinant

    Related Articles

    Recombinant:

    Article Title: An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
    Article Snippet: Mouse monoclonal anti-EN1 , DSHB , 4G11, RRID: AB_528219.

    Knock-Out:

    Article Title: An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
    Article Snippet: Mouse monoclonal anti-EN1 , DSHB , 4G11, RRID: AB_528219.



    Similar Products

    mouse monoclonal anti en1 antibody  (Developmental Studies Hybridoma Bank)
    93
    Developmental Studies Hybridoma Bank mouse monoclonal anti en1 antibody
    Immunofluorescence of hESC-derived mDA progenitors at day 16 (A) LMX1 (floorplate marker) and FOXA2 (ventral midbrain marker), (B) LMX1 and OTX2 (forebrain and midbrain marker), (C) LMX1 and <t>EN1</t> (caudal ventral midbrain marker), (D) LMX1 and NGN2 (neurogenic maker), and (E) SOX2 (neural progenitor marker) and DCX (immature neuronal marker). On day 16, most cells are LMX1 + , FOXA2 + , OTX + 2 and EN1 + mDA progenitors (A-C). Few cells expressed NGN2 (D) and DCX (E). Scale bars, 100 μm.
    Mouse Monoclonal Anti En1 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+en1/anti-Engrail-1/pmc10511858-3-0-6
    Average 93 stars, based on 1 article reviews
    mouse monoclonal anti en1 antibody - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mouse monoclonal anti en1  (Developmental Studies Hybridoma Bank)
    93
    Developmental Studies Hybridoma Bank mouse monoclonal anti en1

    Mouse Monoclonal Anti En1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+en1/anti-Engrail-1/pmc07580226-3-0-4
    Average 93 stars, based on 1 article reviews
    mouse monoclonal anti en1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Immunofluorescence of hESC-derived mDA progenitors at day 16 (A) LMX1 (floorplate marker) and FOXA2 (ventral midbrain marker), (B) LMX1 and OTX2 (forebrain and midbrain marker), (C) LMX1 and EN1 (caudal ventral midbrain marker), (D) LMX1 and NGN2 (neurogenic maker), and (E) SOX2 (neural progenitor marker) and DCX (immature neuronal marker). On day 16, most cells are LMX1 + , FOXA2 + , OTX + 2 and EN1 + mDA progenitors (A-C). Few cells expressed NGN2 (D) and DCX (E). Scale bars, 100 μm.

    Journal: STAR Protocols

    Article Title: A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

    doi: 10.1016/j.xpro.2023.102355

    Figure Lengend Snippet: Immunofluorescence of hESC-derived mDA progenitors at day 16 (A) LMX1 (floorplate marker) and FOXA2 (ventral midbrain marker), (B) LMX1 and OTX2 (forebrain and midbrain marker), (C) LMX1 and EN1 (caudal ventral midbrain marker), (D) LMX1 and NGN2 (neurogenic maker), and (E) SOX2 (neural progenitor marker) and DCX (immature neuronal marker). On day 16, most cells are LMX1 + , FOXA2 + , OTX + 2 and EN1 + mDA progenitors (A-C). Few cells expressed NGN2 (D) and DCX (E). Scale bars, 100 μm.

    Article Snippet: Mouse monoclonal anti-EN1 antibody (1:50) , DSHB , Cat#4G11.

    Techniques: Immunofluorescence, Derivative Assay, Marker

    Immunofluorescence of hESC-derived mDA neurons at day 56 (A–C) (A) FOXA2 and TH (DA neuron marker), (B) NURR1 (DA neuron and post-mitotic DA neuroblast marker) and TH, (C) and EN1 and TH. Scale bars, 200 μm.

    Journal: STAR Protocols

    Article Title: A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

    doi: 10.1016/j.xpro.2023.102355

    Figure Lengend Snippet: Immunofluorescence of hESC-derived mDA neurons at day 56 (A–C) (A) FOXA2 and TH (DA neuron marker), (B) NURR1 (DA neuron and post-mitotic DA neuroblast marker) and TH, (C) and EN1 and TH. Scale bars, 200 μm.

    Article Snippet: Mouse monoclonal anti-EN1 antibody (1:50) , DSHB , Cat#4G11.

    Techniques: Immunofluorescence, Derivative Assay, Marker

    Journal: STAR Protocols

    Article Title: A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

    doi: 10.1016/j.xpro.2023.102355

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-EN1 antibody (1:50) , DSHB , Cat#4G11.

    Techniques: Recombinant

    Journal: STAR Protocols

    Article Title: An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions

    doi: 10.1016/j.xpro.2020.100065

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-EN1 , DSHB , 4G11, RRID: AB_528219.

    Techniques: Recombinant, Knock-Out